anti abcb1 rabbit polyclonal antibody  (Proteintech)

Journal: Journal of Translational Medicine

Article Title: Metabolic reprogramming of poly(morpho)nuclear giant cells determines glioblastoma recovery from doxorubicin-induced stress

doi: 10.1186/s12967-024-05541-9

Figure Lengend Snippet: DOX retention in PGCs. ( a ) Intracellular DOX retention in PGCs following a pulse DOX treatment. DOX-specific fluorescence was registered at the indicated time-points and quantified with immunofluorescence and fluorimetry. ( b ) Perinuclear DOX accumulation in PGCs estimated with ImageStream ® flow-cytometer. ( c ) ImageStream ® (upper panel) and fluorimetric analyses of ABCB1 and ABCG2 levels (lower panel) in T98G cells following their a pulse DOX-treatment. ( d ) Calcein efflux efficiency in PGCs (upper panel) and perinuclear ABCB1/ABCG2 accumulation in PGCs (lower panel) estimated with calcein efflux (vs. control cells (upper left) and SECs (upper right)) and immunofluorescence, respectively. ( e ) A progressive formation of DOXlow SECs in pulse DOX-treated T98G populations visualized by ImageStream. Scale bars = 50 ( a; left , d ) and 10 μm (a; right). Statistical significance of the differences was calculated with t-student test, * p < 0.05 vs. control. Data representative for > 50 cells ( a , c ; lower panels, d ) and > 800 single cells in 3 independent biological replicates ( c ; upper panel, e ). Error bars represent SD ( a , d ) or SEM ( c ) values. Note the expansion of DOXlow population(s) following the DOX retention/compartmentation in PGCs

Article Snippet: Immunolocalization of stress resistance-related proteins was performed with polyclonal rabbit anti-ABCB1 (Sigma; No. HPA002199; 1:250) and monoclonal rabbit anti-ABCG2 (Sigma; No. ZRB1217; 1:100), polyclonal rabbit anti-MnSOD (Sigma; No. HPA001814, 1:300), polyclonal rabbit anti-glutathione synthetase (GSS; ABclonal; No. A14535, 1:200), monoclonal rabbit anti-glycogen synthase kinase-3 beta (GSK-3β) (ABclonal; No. A11731, 1:200) and polyclonal rabbit anti-MTCO2 antibody (Invitrogen; No. MA5-12017; 1:100).

Techniques: Fluorescence, Immunofluorescence, Flow Cytometry, Control

Journal: iScience

Article Title: FAP promotes metastasis and chemoresistance via regulating YAP1 and macrophages in mucinous colorectal adenocarcinoma

doi: 10.1016/j.isci.2023.106600

Figure Lengend Snippet:

Article Snippet: The following primary antibodies were used: rabbit monoclonal anti-human FAP, BMI1, ABCG2, rabbit polyclonal anti-human LMO7, (ab207178, ab126783, ab108312; ab224113; Abcam, Cambridge, UK), rabbit monoclonal anti-human MDR1, NANOG, MPRIP, YAP, pS397YAP, rabbit polyclonal anti-human pS109YAP, KLF4, (13342, 4903, 14396, 14074,13619,46931, 4038; Cell Signaling Technology Inc., Danvers, MA, USA).

Techniques: Virus, Magnetic Beads, Activation Assay, Software